Automation hydrogen/deuterium exchange mass spectrometry, Chemical Crosslinking-MS, structural proteomics, in combination of cell biology, biochemistry and biophysics to resolve important receptor signaling events derived from key diseases mechanisms related to autoimmune diseases and tumors.
1. Nuclear receptors and safer therapeutics targeting osteoporosis and type 2 diabetes. Two first author papers published in Nature Communications and Cell Press Structure.
2. Activation mechanisms of antiviral innate immunity RIG-I like receptors and RIG-I gain-of-function variants involved in autoimmunity. Two first author research articles published in Nucleic Acids Research and Nature Communications.
3. Characterizations of N-glycosylation of viral membrane protein by proteomic studies. One first author research article published in Virology.
4. Applications of hydrogen/deuterium exchange Mass spectrometry; Proteomics. Three first author review articles on applications of Mass spectrometry based approaches.

1.Zheng J, Strutzenberg ST, Bruce Pascal BD, and Griffin PR. Protein dynamics and conformational changes explored by Hydrogen/deuterium exchange Mass spectrometry (In press). Current Opinion of Structure Biology.
2.Zheng J*#, Wang C#, Chang MR, Devarkar SC, Schweibenz B, Crynen GC, Garcia-Ordonez RD, Pascal BD, Novick SJ, Patel SS, Marcotrigiano J*, Griffin PR*. HDX-MS reveals dysregulated checkpoints that compromise discrimination against self RNA during RIG-I mediated autoimmunity. Nature communications. 2018; 9(1):5366.
3.Zheng J#, Corzo C#, Chang MR, Shang J, Lam VQ, Brust R, Blayo AL, Bruning JB, Kamenecka TM, Kojetin DJ, Griffin PR. Chemical Crosslinking Mass Spectrometry Reveals the Conformational Landscape of the Activation Helix of PPARγ; a Model for Ligand-Dependent Antagonism. Structure. 2018; 26(11):1431-1439.e6.
4.Zheng J#, Yamada Y#, Fung TS#, Huang M, Chia R, Liu DX. Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture. Virology. 2018; 513:65-74.
5.Zheng J#, Chang MR, Stites RE, Wang Y, Bruning JB, Pascal BD, Novick SJ, Garcia-Ordonez RD, Stayrook KR, Chalmers MJ, Dodge JA, Griffin PR. HDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions. Nature communications. 2017; 8(1): 923.
6.Zheng J#, Yong HY, Panutdaporn N, Liu C, Tang K, Luo D*. High-resolution HDX-MS reveals distinct mechanisms of RNA recognition and activation by RIG-I and MDA5. Nucleic acids research. 2015; 43(2):1216-30.
7.Zheng J#, Tan BH, Sugrue R, Tang K*. Current approaches on viral infection: proteomics and functional validations. Frontiers in microbiology. 2012; 3:393.
8.Zheng J#, Sugrue RJ, Tang K*. Mass spectrometry based proteomic studies on viruses and hosts--a review. Analytica chimica acta. 2011; 702(2):149-59.